INVIEW Virus Sequencing

Sequencing viruses

Sequencing viruses – Perfect solution for your sample.

RNA & DNA viruses - Choose the perfect NGS solution of your viral sample.

The INVIEW Virus product is the best choice for all sequencing projects, where virus variants need to be determined.

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Applications

Identification of genetic variations, single base mutations, insertions and deletions
Profiling of viral communities
Viral strain identification
Outbreak monitoring
Product safety

Highlights of the INVIEW Virus

Viral samples
DNA virus (dsDNA)
RNA virus (ssRNA or dsRNA)

Product specifications

Processing of any number of samples, starting with one sample
Adaptable sequencing output in multiples of 5M reads
High-quality BioIT analysis
Final report with ready-to-publish data
Available under ISO 17025 accreditation

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Product Specifications & Ordering

Starting material

Starting Material

Accepted starting material for INVIEW Virus

Virus type	Starting material
RNA virus	Type: rRNA depleted or total RNA Quantity: 100 ng per sample Concentration: min. 5 ng/µl Dissolved in: RNase-, DNase- and protease-free molecular grade water
dsDNA virus	600 ng ng gDNA Qubit quantified (up to 200µl / concentration > 3 ng/µl) cDNA can not be accepted as starting material

Accepted starting material for DNA and/or RNA isolation

Tissue
Cells
Blood
Environmental samples
Cell culture supernatant

Please note that only S1-classified material is accepted for DNA / RNA isolation ordered online. More information about the current classification of biological material can be found here (TRBA 466). Please contact us for further information on isolating DNA / RNA from material classified as S2.

Optional pre-sequencing

RNA virus

RNA isolation – efficient isolation of RNA for uniform and less-biased coverage
Ribosomal RNA (rRNA) depletion – optimised removal of rRNA from mRNA for commonly studied organisms

Please contact us to check the compatibility of your organism

DNA virus

DNA isolation – efficient isolation of DNA for uniform and less-biased coverage
Total nucleic acid extraction

Library types

RNA virus

Strand-specific cDNA library. No Poly-A selection.

ds DNA virus

Standard genomic library

Sequencing

Type: Illumina
Run mode: 2 x 150 bp
Output: 5 M read pairs (or multiples of 5M read pairs)

Bioinformatic analysis

Genome variant detection (DNA / RNA virus)

•   Mapping against one reference
•   Detection of SNPs and InDels
•   Annotation of detected SNPs and InDels (using dbSNP)
•   Allocation of effects on protein level (using Ensembl)