Sequencing output is dependent on a variety of factors e.g. organism, extraction method, DNA quality, size, handling & storage. Therefore, successful EntryQC cannot guarantee high sequencing yield as not all inhibitors can be detected.
Plants, especially, can contain high levels of secondary metabolites that can interfere with the sequencing process. These compounds can be challenging to detect and remove during preprocessing, leading to potential issues in sequencing yield and data quality.
Based on our experience, you can generally expect around 50-80 Gb of data per PromethION flow cell and approximately 6-7 Gb per GridION flow cell. These yields are dependent on factors such as DNA quality and fragment length.