The right answers to frequently asked questions
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When should I use the Custom Long-Read Amplicon Sequencing service instead of the ONT Lite Clonal Amplicon Sequencing service?
Custom Long-Read Amplicon Sequencing dedicates an entire flow cell to your amplicons. As a result, this method is more time-consuming and costly compared to ONT Lite Clonal Amplicon Sequencing service, but it is highly suitable in the following cases:
- When you need additional services like 16S Microbiome Analysis, Unique Sequence Analysis, Variant Analysis, Sequence Cleaning, Host Removal, Pod5 raw data delivery
- When your linear DNA sample is not clonal but instead contains a mix of different molecules.
- When full-length, end-to-end reads are required, without any fragmentation.
- When a higher number of sequencing reads is needed beyond the typical output of the ONT Lite Clonal Amplicon Sequencing service.
- When you need to obtain all raw reads generated from your sample.
How should I purify my amplicons?
We recommend purifying your amplicons with commercially available kits based on DNA-binding beads or columns or enzymatic cleanup. DO NOT ship any primers with your samples or mixed into your samples.
What types of samples can be submitted for amplicon sequencing?
Submit purified PCR products with a size range of 500 bp to 25 kb. The amplicons should ideally produce a single band on a gel for optimal results.
Does Eurofins Genomics perform a QC on my samples before library preparation?
Yes, we do perform a QC of the quantity of the amplicons.