IVT-RNA services
In vitro transcription (IVT) - RNAs are used for a variety of applications.
Following IVT-RNA products are possible:
IVT-ssRNA
- Single-stranded RNAs of any scale from 100 bases to several thousand bases
Applications for very long ssRNAs:
- Reference RNA for the development of in vitro diagnostic assays
- Internal control RNA for performance control (e.g. RNA isolation, reverse transcription and qPCR amplification / detection
- Positive control RNAs for controlling high assay qualities
IVT-dsRNA
- Double-stranded RNAs that are synthesised with the ssRNA as a template
- Available in any scale from 100 bp to several thousand base pairs
Applications for dsRNA
- Diagnostic Targets (e.g. Reoviridae with dsRNA genome)
e.g. human rotavirus, sheep and cattle bluetongue virus, human colorado-tick-feaver-virus
- Biological applications
Any kind of application where regulatory RNA or RNAi are needed
IVT-functional mRNA
- Customised mRNA from 1000 to several thousand bases
Possible applications for functional synthetic mRNAs
- Highly specific vaccination
Individualised tumor therapy with tissue-specific and patient-specific tumor markers/antigens (anti-viral therapy)
- Stem cells by cellular reprogramming
For all products a variety of additional services - like GMP-grade synthesis is available.